This data resource contains soil physico-chemical data from eight agricultural sites across the UK sampled during September 2022 and October 2022. For all sites, the data resource presents results for a range of soil characteristics including, pH, soil moisture, stone content, bulk density, organic matter content, soil texture, and total carbon and nitrogen content. Thermo-Gravimetric Analyses (TGA) was performed at one site due to atypically high values for total carbon content. The soil samples were collected using a Split tube sampler. The soil samples were processed at UKCEH laboratories from September 2022 through to August 2023. Sample collection and analysis was performed by project staff. The samples were taken to provide soil baseline data at the eight sites before the planting of perennial biomass crops. The project was funded by the Department for Energy Security & Net Zero (formally the Department for Business, Energy & Industrial Strategy (BEIS)) (Client reference - CEH-303). Full details about this dataset can be found at https://doi.org/10.5285/8c87a397-a010-48a4-8ca5-0f3742c2d857