This is a database of all known birds, bryophytes, fungi, invertebrates, lichens and mammals that use Scots Pine (Pinus sylvestris) in the UK. Scots pine is the UK’s only native conifer that is used commercially and is the most common tree in the UK’s native Caledonian pine woods. In total 1589 species are listed in the database. For each species a level of association with Scots pine is provided, ranging from obligate (only found on Scots pine) to cosmopolitan (found on a wide range of other tree species). Data on the ecology of each Scots pine associated species were collated: part of tree used, and use made of tree (feeding, roosting, breeding). Data on use or otherwise of 34 other tree species were also collated for each of the 1589 species. Full details about this dataset can be found at https://doi.org/10.5285/97f8c19b-7451-4809-b011-0f9cf3eb8430