This dataset contains information about meteorological conditions and ammonia concentration and deposition rates resulting from an experimental setup. An NH3 enhancement experiment along with a full suite of multi-height meteorological measurements was established in a Birch woodland near Edinburgh, UK. Under suitable wind conditions measured at the meteorological tower, NH3 is released towards a monitoring transect. Along the downwind monitoring transect, NH3 concentrations in the air are measured using monthly passive samplers. Deposition rates are modelled using a bi-directional resistance model based on measured NH3 concentrations in the air, micrometeorology and plant physiology. Additionally, NH3 concentrations were measured at high temporal resolution at a fixed downwind distance from the source to achieve the target enhancement concentrations. The work was supported by UKRI GCRF South Asian Nitrogen Hub (Grant NE/S009019/1) and NERC (Grant NE/R016429/1). Full details about this dataset can be found at https://doi.org/10.5285/e30ca77b-9118-4279-8b3a-a4c7773d1c43