[This dataset is embargoed until December 14, 2025]. This data resource contains counts of bumblebee abundance between December 2019 and March 2020 along a latitudinal gradient within Argentina and Chile. Sampling of both invasive (Bombus terrestris and B. ruderatus) and native (B. dahlbomii) bumblebee species was undertaken using standardised transect walks along a latitudinal gradient from -54.871 to -29.906 from 145 individual locations. Full details about this dataset can be found at https://doi.org/10.5285/1797a623-e582-4c0c-bc67-cd9de04e0df0