Dental microwear texture analysis (DMTA) raw data. These are values from scale-sensitive fractal analysis (SSFA), and areal surface texture analysis (based on a suite of parameters, most of which are defined by ISO-25718-2) for dental microwear texture analysis of tooth surfaces of wolves from Britain (MIS 5e & MIS 7 a-c) and modern Poland wolves. This study examines the dietary adaptability of European grey wolves using dental microwear texture analysis (DMTA). We compare modern Polish wolf populations from the present interglacial (Holocene), with fossil specimens from two contrasting Pleistocene interglacials: the warmer Last Interglacial (Marine Oxygen Isotope Stage [MIS] 5e) and the cooler Penultimate Interglacial (MIS 7a–c). These datasets offer new insights into the palaeoclimatic and palaeoenvironmental conditions under which grey wolves endured climate change in the past, free of anthropogenic influence. And allows the identification of hitherto-hidden ecological stress today, thereby highlighting potential vulnerabilities in modern populations under current and future climate change.